Format

Send to

Choose Destination
J Vis Exp. 2015 Sep 22;(103). doi: 10.3791/53156.

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer.

Author information

1
Department of Surgery, Medical University of South Carolina; sdennis@tulane.edu.
2
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina.
3
Department of Bioengineering, Clemson University.
4
Department of Surgery, Medical University of South Carolina.

Abstract

Tissue engineering has centralized its focus on the construction of replacements for non-functional or damaged tissue. The utilization of three-dimensional bioprinting in tissue engineering has generated new methods for the printing of cells and matrix to fabricate biomimetic tissue constructs. The solid freeform fabrication (SFF) method developed for three-dimensional bioprinting uses an additive manufacturing approach by depositing droplets of cells and hydrogels in a layer-by-layer fashion. Bioprinting fabrication is dependent on the specific placement of biological materials into three-dimensional architectures, and the printed constructs should closely mimic the complex organization of cells and extracellular matrices in native tissue. This paper highlights the use of the Palmetto Printer, a Cartesian bioprinter, as well as the process of producing spatially organized, viable constructs while simultaneously allowing control of environmental factors. This methodology utilizes computer-aided design and computer-aided manufacturing to produce these specific and complex geometries. Finally, this approach allows for the reproducible production of fabricated constructs optimized by controllable printing parameters.

PMID:
26436877
PMCID:
PMC4692613
DOI:
10.3791/53156
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for MyJove Corporation Icon for PubMed Central
Loading ...
Support Center