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J Vis Exp. 2015 Sep 22;(103). doi: 10.3791/53156.

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer.

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Department of Surgery, Medical University of South Carolina;
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina.
Department of Bioengineering, Clemson University.
Department of Surgery, Medical University of South Carolina.


Tissue engineering has centralized its focus on the construction of replacements for non-functional or damaged tissue. The utilization of three-dimensional bioprinting in tissue engineering has generated new methods for the printing of cells and matrix to fabricate biomimetic tissue constructs. The solid freeform fabrication (SFF) method developed for three-dimensional bioprinting uses an additive manufacturing approach by depositing droplets of cells and hydrogels in a layer-by-layer fashion. Bioprinting fabrication is dependent on the specific placement of biological materials into three-dimensional architectures, and the printed constructs should closely mimic the complex organization of cells and extracellular matrices in native tissue. This paper highlights the use of the Palmetto Printer, a Cartesian bioprinter, as well as the process of producing spatially organized, viable constructs while simultaneously allowing control of environmental factors. This methodology utilizes computer-aided design and computer-aided manufacturing to produce these specific and complex geometries. Finally, this approach allows for the reproducible production of fabricated constructs optimized by controllable printing parameters.

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