Format

Send to

Choose Destination
Plasmid. 2015 Nov;82:28-34. doi: 10.1016/j.plasmid.2015.10.001. Epub 2015 Oct 3.

Strain ŽP - the first bacterial conjugation-based "kill"-"anti-kill" antimicrobial system.

Author information

1
Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. Electronic address: marjanca.starcic.erjavec@bf.uni-lj.si.
2
Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. Electronic address: ziva.petkovsek@yahoo.com.
3
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Science, 13 Golev Street, 614081 Perm, Russia. Electronic address: mar19719@yandex.ru.
4
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Science, 13 Golev Street, 614081 Perm, Russia. Electronic address: i.maslennikova@rambler.ru.
5
Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. Electronic address: darja.zgur@bf.uni-lj.si.

Abstract

As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of principle that conjugation-based antimicrobial systems are possible. We constructed a bacterial conjugation-based "kill"-"anti-kill" antimicrobial system employing the well known Escherichia coli probiotic strain Nissle 1917 genetically modified to harbor a conjugative plasmid carrying the "kill" gene (colicin ColE7 activity gene) and a chromosomally encoded "anti-kill" gene (ColE7 immunity gene). The constructed strain acts as a donor in conjugal transfer and its efficiency was tested in several types of conjugal assays. Our results clearly demonstrate that conjugation-based antimicrobial systems can be highly efficient.

KEYWORDS:

Bacterial conjugation-based technologies; Bacteriocin; Colicin E7; Escherichia coli; Nissle 1917; Plasmid

PMID:
26436830
DOI:
10.1016/j.plasmid.2015.10.001
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center