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Nat Methods. 2015 Dec;12(12):1132-4. doi: 10.1038/nmeth.3616. Epub 2015 Oct 5.

Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.

Author information

1
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
2
Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.
3
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
4
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
5
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
6
Department of Electrical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
7
The Harvard-MIT Health Sciences and Technology Program, Cambridge, Massachusetts, USA.

Abstract

Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.

PMID:
26436482
PMCID:
PMC4666809
DOI:
10.1038/nmeth.3616
[Indexed for MEDLINE]
Free PMC Article

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