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Nat Methods. 2015 Dec;12(12):1135-8. doi: 10.1038/nmeth.3617. Epub 2015 Oct 5.

A strategy for dissecting the architectures of native macromolecular assemblies.

Author information

  • 1Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
  • 2Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
  • 3Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.
  • 4California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California, USA.
  • 5Institut Pasteur, Paris, France.
  • 6Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.

Abstract

It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

PMID:
26436480
PMCID:
PMC4803312
DOI:
10.1038/nmeth.3617
[PubMed - indexed for MEDLINE]
Free PMC Article
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