Format

Send to

Choose Destination
Genet Mol Res. 2015 Sep 25;14(3):11362-70. doi: 10.4238/2015.September.25.3.

Effect of RAGE polymorphisms on susceptibility to and severity of osteoarthritis in a Han Chinese population: a case-control study.

Author information

1
School of Public Health, National Defense Medical Center, Taipei, Taiwan, China.
2
Department of Orthopedics, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan, China.
3
Department of Family and Community Medicine, Tri-Service General Hospital, Taipei, Taiwan, China.
4
Department of Radiology, Tri-Service General Hospital, Taipei, Taiwan, China.
5
Department of Aviation Medicine and Physical Examination, National Defense Medical Center Tri-Service General Hospital Songshan Branch, Taipei, Taiwan, China.
6
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, China.
7
Department of Nursing, Chang Gung University of Science and Technology, Taoyuan, Taiwan, China.
8
Division of Radiology, National Defense Medical Center Tri-Service General Hospital Songshan Branch, Taipei, Taiwan, China.

Abstract

Recent studies have revealed that the inflammatory process plays a role in the pathogenesis of osteoarthritis (OA). The S100 family and receptor for advanced glycation end products (RAGE) participate in regulating inflammation, even in the production of matrix metalloproteinases (MMPs). MMP-1 degrades cartilage, which may result in OA development. Moreover, polymorphisms in RAGE, S100A8, and MMP-1 have a marked effect on ligand binding and transcription regulating. In this study, we investigated the potential genetic contribution of the RAGE, S100A8, and MMP-1 genes to OA. We performed a matched case-control association study and genotyped OA patients and healthy controls, who were analyzed by polymerase chain reaction-restriction fragment length polymorphism assays. A total of 207 patients were diagnosed with knee OA and underwent total knee replacement. The control group included 207 individuals who had standard X-rays of the knee joints to confirm K/L < 2 and were matched by age and gender. Single-nucleotide polymorphisms in RAGE (-429T/C, -374T/A, and 557G/A), S100A8 (rs3795391A/G), and MMP-1 (-1607 1G/2G, -755G/T, and -519A/G) were evaluated. RAGE -374T/A, S100A8 rs3795391A/G, MMP-1 -1607 1G/2G, -755G/T, and -519A/G showed no significant difference between OA patients and healthy controls. RAGE -429T/C and 557G/A showed a significant association between OA patients and healthy controls (P = 0.016 and 0.047, respectively). In haplotype analyses, no RAGE and MMP-1 haplotypes showed associations with OA. Our results suggest that the investigated polymorphism in the RAGE gene play a role in OA in the Han Chinese population.

PMID:
26436377
DOI:
10.4238/2015.September.25.3
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Fundacao de Pesquisas Cientificas de Ribeirao Preto
Loading ...
Support Center