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Transfusion. 2016 Jan;56(1):244-8. doi: 10.1111/trf.13357. Epub 2015 Oct 4.

Identification of six new RHCE variant alleles in individuals of diverse racial origin.

Author information

1
Canadian Blood Services, Ottawa, Ontario, Canada.
2
Banco de Sangre y Tejidos De Navarra, Pamplona, Spain.
3
Progenika-Grifols, Medford, Massachusetts.
4
Australian Red Cross Blood Service, Brisbane, Australia.
5
Centro de Transfusion de Madrid, Madrid, Spain.
6
LifeShare Blood Centers, Shreveport, Louisiana.
7
Azienda Ospedaliero Universitaria, Udine, Italy.
8
Ospedale Maggiore Policlinico Fondazione IRCCS Ca' Granda, Milano, Italy.
9
Grifols Shared Services, San Marcos, Texas.

Abstract

BACKGROUND:

The introduction of molecular methods into routine blood typing is prompting the identification of new blood group alleles. Discrepancies between the results of genotyping and serology or chance events uncovered during genotyping prompted additional investigations, which revealed six new RHCE variant alleles.

STUDY DESIGN AND METHODS:

Samples from eight blood donors, two patients (one prenatal), and a patient's relative, all of diverse racial origin, were analyzed by standard serology methods, targeted genotyping arrays, DNA sequencing, and allele-specific polymerase chain reaction.

RESULTS:

Six new RHCE alleles were identified, namely, RHCE*cE84A, RHCE*ce202G, RHCE*ce307T, RHCE*Ce377G, RHCE*ce697G,712G,733G,744C, and RHCE*Ce733G.

CONCLUSION:

While implementation of new assays in commercial genotyping platforms to detect the polymorphisms reported here may not be justified given their apparent rarity, software interpretative algorithms may benefit from the identification of new alleles for a more accurate determination of genotypes and prediction of phenotypes.

PMID:
26435076
DOI:
10.1111/trf.13357
[Indexed for MEDLINE]

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