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J Allergy Clin Immunol. 2016 Feb;137(2):507-516.e8. doi: 10.1016/j.jaci.2015.08.016. Epub 2015 Oct 1.

Extracellular vesicle-derived protein from Bifidobacterium longum alleviates food allergy through mast cell suppression.

Author information

1
Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea; Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea.
2
Division of Molecular and Life Science, Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea.
3
Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea.
4
National Institute of Animal Science, Rural Development Administration, Jeonju, Korea.
5
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea; Division of Molecular and Life Science, Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea.
6
Department of Microbiology, Ewha Womans University Graduate School of Medicine, Seoul, Korea.
7
Department of Medicine, Ewha Womans University Graduate School of Medicine, Seoul, Korea.
8
Faculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju, Korea.
9
National Institute of Animal Science, Rural Development Administration, Jeonju, Korea. Electronic address: hamjs@korea.kr.
10
Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea. Electronic address: yangbg@ibs.re.kr.
11
Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea. Electronic address: jangmh@ibs.re.kr.

Abstract

BACKGROUND:

The incidence of food allergies has increased dramatically during the last decade. Recently, probiotics have been studied for the prevention and treatment of allergic disease.

OBJECTIVE:

We examined whether Bifidobacterium longum KACC 91563 and Enterococcus faecalis KACC 91532 have the capacity to suppress food allergies.

METHODS:

B longum KACC 91563 and E faecalis KACC 91532 were administered to BALB/c wild-type mice, in which food allergy was induced by using ovalbumin and alum. Food allergy symptoms and various immune responses were assessed.

RESULTS:

B longum KACC 91563, but not E faecalis KACC 91532, alleviated food allergy symptoms. Extracellular vesicles of B longum KACC 91563 bound specifically to mast cells and induced apoptosis without affecting T-cell immune responses. Furthermore, injection of family 5 extracellular solute-binding protein, a main component of extracellular vesicles, into mice markedly reduced the occurrence of diarrhea in a mouse food allergy model.

CONCLUSION:

B longum KACC 91563 induces apoptosis of mast cells specifically and alleviates food allergy symptoms. Accordingly, B longum KACC 91563 and family 5 extracellular solute-binding protein exhibit potential as therapeutic approaches for food allergies.

KEYWORDS:

Bifidobacterium longum; Food allergy; extracellular vesicle; family 5 extracellular solute-binding protein; mast cells; ovalbumin; probiotics

PMID:
26433560
DOI:
10.1016/j.jaci.2015.08.016
[Indexed for MEDLINE]

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