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Pathophysiology. 2015 Dec;22(4):241-8. doi: 10.1016/j.pathophys.2015.09.003. Epub 2015 Sep 28.

Vitamins C and E (ascorbate/α-tocopherol) provide synergistic neuroprotection in the jejunum in experimental diabetes.

Author information

1
Department of Health and Education, Universidade Estadual do Norte do Paraná, 86360-000 Paraná, Brazil.
2
Universidade Federal de Mato Grosso do Sul, 79400-000 Coxim, Mato Grosso do Sul, Brazil.
3
Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil.
4
Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil. Electronic address: jnzanoni@uem.br.

Abstract

The present study evaluated the synergistic effects of the association of ascorbic acid and α-tocopherol on myenteric in the jejunum of diabetic rats. The rats were randomly divided into four equal groups: untreated normoglycemic (UC), untreated diabetic (UD), ascorbic acid and α-tocopherol-treated normoglycemic (CAE) and ascorbic acid and α-tocopherol-treated diabetic (DAE). The rats from the CAE and DAE group received supplementation with ascorbic acid (1g/L in water) and α-tocopherol (1% in chow). At 210-days-old, the animals were sacrified and their jejunum was collected and submitted to immunohistochemistry. Quantitative and/or morphometric analysis were performed. Supplementation with ascorbic acid and α-tocopherol prevented the cell loss of myenteric neurons expressing HuC/D and TrkA in an equivalent proportion. We also observed a reduction of the CGRP nerve fiber varicosities and the prevention of the increased cell body size of submucosal VIP neurons (p<0.05). The association of ascorbic acid and α-tocopherol reduced the deleterious effects of diabetes promoting protection on the enteric neurons.

KEYWORDS:

Antioxidant; Diabetes mellitus; Enteric nervous system; Neuropathy; Oxidative stress; Supplementation

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