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Virology. 2015 Dec;486:116-20. doi: 10.1016/j.virol.2015.08.002. Epub 2015 Oct 1.

Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers.

Author information

1
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. Electronic address: k.h.sliepen@amc.uva.nl.
2
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.m.medinaramirez@amc.uva.nl.
3
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: yasmeenanila1@gmail.com.
4
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: pek2003@med.cornell.edu.
5
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: jpm2003@med.cornell.edu.
6
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065, USA. Electronic address: r.w.sanders@amc.uva.nl.

Abstract

HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 inferred germline precursors of bNAbs directed to different epitope clusters to three soluble native-like SOSIP.664 Env trimers. We found that native-like SOSIP.664 trimers bind to some inferred germline precursors of bNAbs, particularly ones involving the V1/V2 loops at the apex of the trimer. The data imply that native-like SOSIP.664 trimers will be an appropriate platform for structure-guided design improvements intended to create immunogens able to target the germline precursors of bNAbs.

KEYWORDS:

Broadly neutralizing antibodies; Env; Germline; HIV-1; SOSIP; Vaccine

PMID:
26433050
PMCID:
PMC4712445
DOI:
10.1016/j.virol.2015.08.002
[Indexed for MEDLINE]
Free PMC Article

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