Quantification of bovine oxylipids during intramammary Streptococcus uberis infection

V E Ryman; G M Pighetti; J D Lippolis; J C Gandy; C M Applegate; L M Sordillo

doi:10.1016/j.prostaglandins.2015.09.006

Quantification of bovine oxylipids during intramammary Streptococcus uberis infection

Prostaglandins Other Lipid Mediat. 2015 Sep;121(Pt B):207-17. doi: 10.1016/j.prostaglandins.2015.09.006. Epub 2015 Sep 30.

Authors

V E Ryman¹, G M Pighetti², J D Lippolis³, J C Gandy¹, C M Applegate¹, L M Sordillo⁴

Affiliations

¹ College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
² Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA.
³ Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, USDA-Agricultural Research Service, Ames, IA 50010, USA.
⁴ College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. Electronic address: sordillo@msu.edu.

PMID: 26432060
DOI: 10.1016/j.prostaglandins.2015.09.006

Abstract

Streptococcus uberis mastitis results in severe mammary tissue damage in dairy cows due to uncontrolled inflammation. Oxylipids are potent lipid mediators that orchestrate pathogen-induced inflammatory responses, however, changes in oxylipid biosynthesis during S. uberis mastitis are unknown. Thus, the current objective was to determine how oxylipid concentrations change in milk and mammary tissues during different stages of S. uberis mastitis. Increased arachidonic acid and linoleic acid-derived oxylipids were significantly increased in S. uberis-infected bovine mammary tissue. Linoleic acid metabolites, hydroxyoctadecadienoic acid (HODE) and oxooctadecadienoic acid, predominated in tissue and milk. Furthermore, in vitro exposure of bovine mammary endothelial cells to 13-hydroperoxyoctadecadienoic acid, upstream metabolite of HODE, significantly increased cyclooxygenase-2 expression, but 13-HODE exposure had no effect. The findings in the current study indicate lipidomic profiling may explain some of the dynamics of inflammation during bacterial challenge, however continued research is necessary to determine sample compartments which best reflect disease pathogenesis.

Keywords: Cyclooxygenase; Eicosanoid; Inflammation; Lipoxygenase; Mastitis; Oxylipids.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Animals, Inbred Strains
Cattle
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / metabolism
Cells, Cultured
Cyclooxygenase 2 / chemistry
Cyclooxygenase 2 / genetics
Cyclooxygenase 2 / metabolism
Cytokines / genetics
Cytokines / metabolism
Dairying
Eicosanoids / analysis
Eicosanoids / metabolism*
Endothelium, Vascular / cytology
Endothelium, Vascular / immunology
Endothelium, Vascular / metabolism
Endothelium, Vascular / pathology
Female
Host-Pathogen Interactions*
Linoleic Acids / analysis
Linoleic Acids / metabolism
Mammary Glands, Animal / immunology
Mammary Glands, Animal / metabolism*
Mammary Glands, Animal / microbiology
Mammary Glands, Animal / pathology
Mastitis, Bovine / immunology
Mastitis, Bovine / metabolism*
Mastitis, Bovine / microbiology
Mastitis, Bovine / physiopathology
Milk / chemistry*
Milk / microbiology
RNA, Messenger / metabolism
Streptococcal Infections / immunology
Streptococcal Infections / metabolism
Streptococcal Infections / microbiology
Streptococcal Infections / veterinary*
Streptococcus / immunology
Streptococcus / isolation & purification
Streptococcus / physiology*
Up-Regulation

Substances

Cell Adhesion Molecules
Cytokines
Eicosanoids
Linoleic Acids
RNA, Messenger
Cyclooxygenase 2
13-hydroperoxylinoleic acid