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Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):E5583-9. doi: 10.1073/pnas.1513882112. Epub 2015 Oct 1.

Regulation of outer kinetochore Ndc80 complex-based microtubule attachments by the central kinetochore Mis12/MIND complex.

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Department of Biochemistry, University of Washington, Seattle, WA 98195;
Department of Genome Sciences, University of Washington, Seattle, WA 98195;
Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195.
Department of Biochemistry, University of Washington, Seattle, WA 98195;


Multiple protein subcomplexes of the kinetochore cooperate as a cohesive molecular unit that forms load-bearing microtubule attachments that drive mitotic chromosome movements. There is intriguing evidence suggesting that central kinetochore components influence kinetochore-microtubule attachment, but the mechanism remains unclear. Here, we find that the conserved Mis12/MIND (Mtw1, Nsl1, Nnf1, Dsn1) and Ndc80 (Ndc80, Nuf2, Spc24, Spc25) complexes are connected by an extensive network of contacts, each essential for viability in cells, and collectively able to withstand substantial tensile load. Using a single-molecule approach, we demonstrate that an individual MIND complex enhances the microtubule-binding affinity of a single Ndc80 complex by fourfold. MIND itself does not bind microtubules. Instead, MIND binds Ndc80 complex far from the microtubule-binding domain and confers increased microtubule interaction of the complex. In addition, MIND activation is redundant with the effects of a mutation in Ndc80 that might alter its ability to adopt a folded conformation. Together, our results suggest a previously unidentified mechanism for regulating microtubule binding of an outer kinetochore component by a central kinetochore complex.


MIND/Mis12 complex; Ndc80 complex; kinetochore; microtubules; mitosis

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