Format

Send to

Choose Destination
Science. 2015 Oct 30;350(6260):563-567. doi: 10.1126/science.aab2749. Epub 2015 Oct 1.

Retroviruses use CD169-mediated trans-infection of permissive lymphocytes to establish infection.

Author information

1
Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06510, USA.
2
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
3
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA.
4
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
5
Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
6
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA.
7
The Jackson Laboratory, Bar Harbor, ME 04609, USA..
#
Contributed equally

Abstract

Dendritic cells can capture and transfer retroviruses in vitro across synaptic cell-cell contacts to uninfected cells, a process called trans-infection. Whether trans-infection contributes to retroviral spread in vivo remains unknown. Here, we visualize how retroviruses disseminate in secondary lymphoid tissues of living mice. We demonstrate that murine leukemia virus (MLV) and human immunodeficiency virus (HIV) are first captured by sinus-lining macrophages. CD169/Siglec-1, an I-type lectin that recognizes gangliosides, captures the virus. MLV-laden macrophages then form long-lived synaptic contacts to trans-infect B-1 cells. Infected B-1 cells subsequently migrate into the lymph node to spread the infection through virological synapses. Robust infection in lymph nodes and spleen requires CD169, suggesting that a combination of fluid-based movement followed by CD169-dependent trans-infection can contribute to viral spread.

Comment in

PMID:
26429886
PMCID:
PMC4651917
DOI:
10.1126/science.aab2749
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center