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Biotechnol Lett. 2016 Jan;38(1):43-50. doi: 10.1007/s10529-015-1964-4. Epub 2015 Oct 1.

Antitumor activity of ginseng sapogenins, 25-OH-PPD and 25-OCH3-PPD, on gastric cancer cells.

Author information

1
Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
2
Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China. zyq4885@126.com.

Abstract

OBJECTIVES:

25-Hydroxyprotopanaxadiol (25-OH-PPD) and 25-methoxylprotopanaxadiol (25-OCH3-PPD), two ginseng sapogenins, have potent antitumor activity and their effects on gastric cancer (BGC-823, SGC-7901, MKN-28) cells and a gastric mucosa (GES-1) cell line are reported.

RESULTS:

Both compounds significantly inhibited the growth of gastric cancer cells, while having lesser inhibitory effects on GES-1 cells by MTT assay. A mechanistic study revealed that the two ginseng sapogenins could induce apoptosis in BGC-823 cells by morphological observation, DNA fragmentation, flow cytometry and western blot analysis. Besides, the apoptosis was inhibited by Ac-DEVD-CHO, a caspase 3 inhibitor, which was confirmed by cell viability analysis.

CONCLUSIONS:

These results indicate that 25-OH-PPD and 25-OCH3-PPD have potential to be promising agents for the treatment of gastric cancer.

KEYWORDS:

25-Hydroxyprotopanaxadiol; 25-Methoxylprotopanaxadiol; Antitumor activity; Apoptosis; Gastric cancer; Ginseng sapogenins

PMID:
26428367
DOI:
10.1007/s10529-015-1964-4
[Indexed for MEDLINE]

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