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Biomed Res Int. 2015;2015:730390. doi: 10.1155/2015/730390. Epub 2015 Sep 3.

Expression of Translationally Controlled Tumor Protein in Human Kidney and in Renal Cell Carcinoma.

Author information

1
Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
2
Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy ; Section of Pathology, Ospedale di Circolo di Busto Arsizio, Presidio Ospedaliero di Saronno, Piazzale Borella 1, 21047 Saronno, Italy.
3
Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy ; Section of Pathology, Azienda Ospedaliera, "G. Salvini", Viale C. Forlanini 121, 20024 Garbagnate Milanese, Italy.
4
Section of Pathology, Ospedale di Circolo di Busto Arsizio, Presidio Ospedaliero di Saronno, Piazzale Borella 1, 21047 Saronno, Italy.
5
Section of Pathology, Azienda Ospedaliera, "G. Salvini", Viale C. Forlanini 121, 20024 Garbagnate Milanese, Italy.
6
Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy ; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E14NS, UK.
7
Department of Medicine, Science and Neurosciences, University of Siena, Via delle Scotte 6, 53100 Siena, Italy.
8
Section of Pathology, Azienda Ospedaliera Universitaria Senese, Viale Bracci 16, 53100 Siena, Italy.

Abstract

Translationally controlled tumor protein is a multifaceted protein involved in several physiological and biological functions. Its expression in normal kidney and in renal carcinomas, once corroborated by functional data, may add elements to elucidate renal physiology and carcinogenesis. In this study, translationally controlled tumor protein expression was evaluated by quantitative real time polymerase chain reaction and western blotting, and its localization was examined by immunohistochemistry on 84 nephrectomies for cancer. In normal kidney protein expression was found in the cytoplasm of proximal and distal tubular cells, in cells of the thick segment of the loop of Henle, and in urothelial cells of the pelvis. It was also detectable in cells of renal carcinoma with different pattern of localization (membranous and cytoplasmic) depending on tumor histotype. Our data may suggest an involvement of translationally controlled tumor protein in normal physiology and carcinogenesis. However, functional in vitro and in vivo studies are needed to verify this hypothesis.

PMID:
26425551
PMCID:
PMC4573619
DOI:
10.1155/2015/730390
[Indexed for MEDLINE]
Free PMC Article

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