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Methods Mol Biol. 2015;1327:141-58. doi: 10.1007/978-1-4939-2842-2_11.

Electron Tomography Methods for C. elegans.

Author information

1
Albert Einstein College of Medicine, Center for C. elegans Anatomy, 1410 Pelham Parkway South, Room 601, Bronx, NY, 10461, USA. david.hall@einstein.yu.edu.
2
New York Structural Biology Center, New York, NY, USA. rice@nysbc.org.

Abstract

Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fiducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.

KEYWORDS:

Annotation; Back-projection; Denoising; Electron tomogram; Fiducial marker; High pressure freezing; Image acquisition; Image alignment; Markerless alignment

PMID:
26423973
DOI:
10.1007/978-1-4939-2842-2_11
[Indexed for MEDLINE]

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