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Methods Mol Biol. 2015;1327:59-74. doi: 10.1007/978-1-4939-2842-2_6.

Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System.

Author information

1
FAS Center for Systems Biology, Harvard University, Northwest Lab Building, 52 Oxford Street, B227.80, Cambridge, MA, 02138, USA. jcalarco@fas.harvard.edu.
2
Editas Medicine, Cambridge, MA, USA.

Abstract

The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.

KEYWORDS:

C. elegans; CRISPR; Cas9; Genome editing; Genome engineering

PMID:
26423968
DOI:
10.1007/978-1-4939-2842-2_6
[Indexed for MEDLINE]

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