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Nat Commun. 2015 Oct 1;6:8497. doi: 10.1038/ncomms9497.

SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.

Author information

1
Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
2
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
3
Department of Chemistry-BMC, Uppsala University, 75123 Uppsala, Sweden.
4
Department of Biochemistry, NCCR in Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.
5
Department NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Abstract

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

PMID:
26423723
PMCID:
PMC4600740
DOI:
10.1038/ncomms9497
[Indexed for MEDLINE]
Free PMC Article

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