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Stem Cell Res Ther. 2015 Sep 29;6:188. doi: 10.1186/s13287-015-0163-5.

Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration.

Chabot V1, Dromard C2,3,4,5, Rico A6, Langonné A7, Gaillard J8,9, Guilloton F10,11,12,13, Casteilla L14,15,16,17, Sensebé L18,19,20,21.

Author information

1
EFS Centre-Atlantique, BP 40661, 37 206, Tours, Cedex 3, France. valerie.chabot@efs.sante.fr.
2
CNRS UMR5273 STROMALab, BP 84225, F-31 432, Toulouse, Cedex 4, France. cecile.berthezene@inserm.fr.
3
Université Paul Sabatier de Toulouse, BP 84225, F-31 432, Toulouse, Cedex 4, France. cecile.berthezene@inserm.fr.
4
INSERM U1031, BP 84225, F-31 432, Toulouse, Cedex 4, France. cecile.berthezene@inserm.fr.
5
EFS Pyrénées -Méditerranée BP 84225, F-31 432, Toulouse, Cedex 4, France. cecile.berthezene@inserm.fr.
6
EFS Centre-Atlantique, BP 40661, 37 206, Tours, Cedex 3, France. angelique.rico@efs.sante.fr.
7
EFS Centre-Atlantique, BP 40661, 37 206, Tours, Cedex 3, France. alain.langonne@efs.sante.fr.
8
EFS Centre-Atlantique, BP 40661, 37 206, Tours, Cedex 3, France. julien.gaillard@univ-tours.fr.
9
Département des Microscopies, Faculté de Médecine, 37 032, Tours, Cedex, France. julien.gaillard@univ-tours.fr.
10
CNRS UMR5273 STROMALab, BP 84225, F-31 432, Toulouse, Cedex 4, France. fabien.guilloton@efs.sante.fr.
11
Université Paul Sabatier de Toulouse, BP 84225, F-31 432, Toulouse, Cedex 4, France. fabien.guilloton@efs.sante.fr.
12
INSERM U1031, BP 84225, F-31 432, Toulouse, Cedex 4, France. fabien.guilloton@efs.sante.fr.
13
EFS Pyrénées -Méditerranée BP 84225, F-31 432, Toulouse, Cedex 4, France. fabien.guilloton@efs.sante.fr.
14
CNRS UMR5273 STROMALab, BP 84225, F-31 432, Toulouse, Cedex 4, France. louis.casteilla@inserm.fr.
15
Université Paul Sabatier de Toulouse, BP 84225, F-31 432, Toulouse, Cedex 4, France. louis.casteilla@inserm.fr.
16
INSERM U1031, BP 84225, F-31 432, Toulouse, Cedex 4, France. louis.casteilla@inserm.fr.
17
EFS Pyrénées -Méditerranée BP 84225, F-31 432, Toulouse, Cedex 4, France. louis.casteilla@inserm.fr.
18
CNRS UMR5273 STROMALab, BP 84225, F-31 432, Toulouse, Cedex 4, France. luc.sensebe@efs.sante.fr.
19
Université Paul Sabatier de Toulouse, BP 84225, F-31 432, Toulouse, Cedex 4, France. luc.sensebe@efs.sante.fr.
20
INSERM U1031, BP 84225, F-31 432, Toulouse, Cedex 4, France. luc.sensebe@efs.sante.fr.
21
EFS Pyrénées -Méditerranée BP 84225, F-31 432, Toulouse, Cedex 4, France. luc.sensebe@efs.sante.fr.

Abstract

INTRODUCTION:

Mesenchymal stem cells (MSC) are well described for their role in tissue regeneration following injury. Migratory properties of endogenous or administrated MSC are critical for tissue repair processes. Platelet-derived growth factor (PDGF) is a chemotactic growth factor that elicits mesenchymal cell migration. However, it is yet to be elucidated if signaling pathways other than direct activation of PDGF receptor (PDGF-R) are involved in PDGF-induced cell migration.

METHODS:

Knocking down and co-immunoprecipitation approaches were used to evaluate urokinase-type plasminogen activator receptor (uPAR) requirement and its interactions with proteins involved in migration mechanisms, in human MSC induced to migrate under PDGF-AB effect.

RESULTS:

We demonstrated that uPAR activation and its association with β1-integrin are required for PDGF-AB-induced migration. This phenomenon takes place in MSC derived from bone marrow and from adipose tissue.

CONCLUSIONS:

We showed that PDGF-AB downstream signaling requires other effector molecules in MSC such as the uPA/uPAR system and β1 integrin signaling pathway known for their role in migration. These findings provide new insights in molecular mechanisms of PDGF-AB-induced migration of human MSC that may be relevant to control MSC function and tissue remodeling after injury.

PMID:
26420039
PMCID:
PMC4588680
DOI:
10.1186/s13287-015-0163-5
[Indexed for MEDLINE]
Free PMC Article

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