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G3 (Bethesda). 2015 Sep 28;5(12):2539-45. doi: 10.1534/g3.115.020057.

Genome Sequences of Three Phytopathogenic Species of the Magnaporthaceae Family of Fungi.

Author information

1
Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606.
2
Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606.
3
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003.
4
The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
5
Department of Plant Pathology, Ohio State University, Columbus, Ohio 43210.
6
USDA-ARS, Root Disease and Biological Control, Pullman, Washington 99164.
7
Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546.
8
Department of Biology, University of Toronto, Mississauga, Toronto, Ontario, L5L 1C6, Canada.
9
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003 radean2@ncsu.edu lijun@biochem.umass.edu.
10
Center for Integrated Fungal Research, North Carolina State University, Raleigh, North Carolina 27606 Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27606 radean2@ncsu.edu lijun@biochem.umass.edu.

Abstract

Magnaporthaceae is a family of ascomycetes that includes three fungi of great economic importance: Magnaporthe oryzae, Gaeumannomyces graminis var. tritici, and Magnaporthe poae. These three fungi cause widespread disease and loss in cereal and grass crops, including rice blast disease (M. oryzae), take-all disease in wheat and other grasses (G. graminis), and summer patch disease in turf grasses (M. poae). Here, we present the finished genome sequence for M. oryzae and draft sequences for M. poae and G. graminis var. tritici. We used multiple technologies to sequence and annotate the genomes of M. oryzae, M. poae, and G. graminis var. tritici. The M. oryzae genome is now finished to seven chromosomes whereas M. poae and G. graminis var. tritici are sequenced to 40.0× and 25.0× coverage respectively. Gene models were developed by the use of multiple computational techniques and further supported by RNAseq data. In addition, we performed preliminary analysis of genome architecture and repetitive element DNA.

KEYWORDS:

Gaeumannomyces; Magnaporthe; repetitive DNA; sequence; synteny

PMID:
26416668
PMCID:
PMC4683626
DOI:
10.1534/g3.115.020057
[Indexed for MEDLINE]
Free PMC Article

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