(a) Modified box-and-whiskers graph for the microtubule growth speeds in the absence (control) and presence of different concentrations of full-length mGFP-TPX2 and mGFP-TPX2mini, as indicated. Number of observed microtubule growth episodes per condition: control – n=150; mGFP-TPX2: 0.2 nM – n= 160, 1 nM – n=150, 5 nM – n=114; mGFP-TPX2mini: 10 nM – n=153, 50 nM – n=121, 250 nM – n=154. All events are from one dataset each. (b) TIRF microscopy kymographs showing dynamic Atto647N-labelled microtubules in the absence (control) or presence of 5 nM mGFP-TPX2 or 250 nM mGFP-TPX2mini. Scale bars as indicated. Bar graphs showing microtubule (c) catastrophe and (d) rescue frequencies, and box-and-whiskers graphs showing microtubule (e) growth and (f) depolymerisation speeds, for control, 5 nM mGFP-TPX2, and 250 nM mGFP-TPX2mini, as indicated. Box-and-whiskers graphs show (g) microtubule lifetimes, (h) depolymerisation times and (i) depolymerisation lengths for the same conditions. Data for (c-i) were pooled from three datasets each. Total number of analysed events per condition: (c) catastrophes (total growth time in brackets): control – n=757 (457,280 s), mGFP-TPX2 –n= 209 (267,644 s), mGFP-TPX2mini – n=385 (409,675 s); (d) rescues (total depolymerisation time in brackets): control – n=612 (22,062 s), mGFP-TPX2 – n=209 (10,708 s), mGFP-TPX2mini – n=376 (13,130 s); (e) growth episodes: control – n=863, 5 nM mGFP-TPX2 – n=271, 250 nM mGFP-TPX2mini –n=477; (f) depolymerisation episodes: control – n=756, 5 nM, mGFP-TPX2 – n=211, 250 nM mGFP-TPX2mini –n=383. The same data were used in (g)-(i) (see ). Atto647N-labelled tubulin concentration was always 7.5 μM. Errors in bar graphs are SEM. For the modified box-and-whiskers plots the boxes range from 25th to 75th percentile, the whiskers span from 10th to 90th percentile, the horizontal line marks the mean value. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 (only displayed for comparisons with control); determined for the comparison of mean values analysing raw data (Tukey’s test in conjunction with One Way ANOVA).