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Braz J Microbiol. 2015 Jul 1;46(3):861-5. doi: 10.1590/S1517-838246320140051. eCollection 2015 Jul-Sep.

Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov.

Author information

1
Mumbai Research Centre, Central Institute of Fisheries Technology, Mumbai, India .
2
Veterinary College and Research Institute, Tamil Nadu, India .
3
Central Institute of Brackishwater Aquaculture, Tamil Nadu, India .
4
Veterinary College, Tamil Nadu, India .

Abstract

Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18(th)(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.

KEYWORDS:

Komarov strain; Newcastle disease; Newcastle disease vaccine; chicken embryo fibroblast

PMID:
26413071
PMCID:
PMC4568861
DOI:
10.1590/S1517-838246320140051
[Indexed for MEDLINE]
Free PMC Article

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