Quercetin promotes neurite growth through enhancing intracellular cAMP level and GAP-43 expression

Chin J Nat Med. 2015 Sep;13(9):667-72. doi: 10.1016/S1875-5364(15)30064-9.

Abstract

The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE(tm) cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular cAMP level and Gap-43 expression.

Keywords: Gap-43; N1E-115; Quercetin; cAMP; neurite growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cyclic AMP / metabolism*
  • GAP-43 Protein / metabolism*
  • Nerve Regeneration
  • Neurites / drug effects*
  • Plant Extracts / pharmacology*
  • Quercetin / pharmacology*
  • Signal Transduction

Substances

  • GAP-43 Protein
  • Plant Extracts
  • Quercetin
  • Cyclic AMP