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Cell. 2015 Oct 8;163(2):367-80. doi: 10.1016/j.cell.2015.08.058. Epub 2015 Sep 24.

Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells.

Author information

1
RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
2
RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
3
Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
4
Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
5
Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8561, Japan.
6
Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan; School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
7
Center for Sustainable Resource Science (CSRS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
8
Laboratory of Microbiology and Immunology, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, 1-25-4 Daigaku-Nishi, Gifu 501-1196, Japan.
9
School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
10
School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan; CREST, JST, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan.
11
Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA.
12
Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
13
Department of Evolutionary Genetics, Max Planck Institute of Evolutionary Anthropology, Deutscher Platz 6, 04103 Leipzig, Germany.
14
Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan. Electronic address: yoshinori-umesaki@yakult.co.jp.
15
RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; CREST, JST, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan. Electronic address: kenya@keio.jp.

Abstract

Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.

PMID:
26411289
PMCID:
PMC4765954
DOI:
10.1016/j.cell.2015.08.058
[Indexed for MEDLINE]
Free PMC Article

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