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Sci Rep. 2015 Sep 28;5:14437. doi: 10.1038/srep14437.

Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins.

Author information

1
Center for Biochemistry, Medical Faculty, Center for Molecular Medicine Cologne (CMMC) and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Köln, Germany.
2
Institute of Anatomy and Cell Biology, Ludwig-Maximilians-University, 80336 München.
3
Department of Biophysical Chemistry, Medizinische Hochschule Hannover, 30623 Hannover, Germany.

Abstract

Coronin7 (CRN7) stabilizes F-actin and is a regulator of processes associated with the actin cytoskeleton. Its loss leads to defects in phagocytosis, motility and development. It harbors a CRIB (Cdc42- and Rac-interactive binding) domain in each of its WD repeat domains which bind to Rac GTPases preferably in their GDP-loaded forms. Expression of wild type CRN7 in CRN7 deficient cells rescued these defects, whereas proteins with mutations in the CRIB motifs which were associated with altered Rac binding were effective to varying degrees. The presence of one functional CRIB was sufficient to reestablish phagocytosis, cell motility and development. Furthermore, by molecular modeling and mutational analysis we identified the contact regions between CRN7 and the GTPases. We also identified WASP, SCAR and PAKa as downstream effectors in phagocytosis, development and cell surface adhesion, respectively, since ectopic expression rescued these functions.

PMID:
26411260
PMCID:
PMC4585930
DOI:
10.1038/srep14437
[Indexed for MEDLINE]
Free PMC Article

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