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Retrovirology. 2015 Sep 26;12:82. doi: 10.1186/s12977-015-0210-4.

Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity.

Author information

1
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands. k.h.sliepen@amc.uva.nl.
2
Department of Integrative Structural and Computational Biology, IAVI Neutralizing Antibody Center, Collaboration for AIDS Vaccine Discovery (CAVD), Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA, 92037, USA. gozorows@scripps.edu.
3
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands. j.a.burger@amc.uva.nl.
4
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands. t.vanmontfort@amc.uva.nl.
5
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands. melissa.stunnenberg@student.uva.nl.
6
Department of Surgery, Duke University Medical Center, Durham, NC, 27710, USA. celia.labranche@dm.duke.edu.
7
Department of Surgery, Duke University Medical Center, Durham, NC, 27710, USA. david.montefiori@duke.edu.
8
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY, 10065, USA. jpm2003@med.cornell.edu.
9
Department of Integrative Structural and Computational Biology, IAVI Neutralizing Antibody Center, Collaboration for AIDS Vaccine Discovery (CAVD), Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA, 92037, USA. abward@scripps.edu.
10
Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands. r.w.sanders@amc.uva.nl.
11
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY, 10065, USA. r.w.sanders@amc.uva.nl.

Abstract

BACKGROUND:

Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation.

FINDINGS:

We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses (but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins.

CONCLUSIONS:

This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers.

PMID:
26410741
PMCID:
PMC4583754
DOI:
10.1186/s12977-015-0210-4
[Indexed for MEDLINE]
Free PMC Article

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