Send to

Choose Destination
J Agric Food Chem. 2015 Oct 14;63(40):8930-9. doi: 10.1021/acs.jafc.5b04388. Epub 2015 Oct 2.

HPLC Separation of Vitamin E and Its Oxidation Products and Effects of Oxidized Tocotrienols on the Viability of MCF-7 Breast Cancer Cells in Vitro.

Author information

Department of Analytical Chemistry and ‡Department of Food Toxicology, Center for Food Sciences, Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover Foundation , Bischofsholer Damm 15, D-30173 Hannover, Germany.


Tocotrienols, a vitamin E subgroup, exert potent anticancer effects, but easily degrade due to oxidation. Eight vitamin E reference compounds, α-, β-, γ-, or δ-tocopherols or -tocotrienols, were thermally oxidized in n-hexane. The corresponding predominantly dimeric oxidation products were separated from the parent compounds by diol-modified normal-phase HPLC-UV and characterized by mass spectroscopy. The composition of test compounds, that is, α-tocotrienol, γ-tocotrienol, or palm tocotrienol-rich fraction (TRF), before and after thermal oxidation was determined by HPLC-DAD, and MCF-7 cells were treated with both nonoxidized and oxidized test compounds for 72 h. Whereas all nonoxidized test compounds (0-100 μM) led to dose-dependent decreases in cell viability, equimolar oxidized α-tocotrienol had a weaker effect, and oxidized TRF had no such effect. However, the IC50 value of oxidized γ-tocotrienol was lower (85 μM) than that of nonoxidized γ-tocotrienol (134 μM), thereby suggesting that γ-tocotrienol oxidation products are able to reduce tumor cell viability in vitro.


HPLC-DAD; alamarBlue assay; dimeric oxidation products; human MCF-7 breast cancer cells; tocopherols; tocotrienols; viability; vitamin E

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center