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J Med Microbiol. 2015 Nov;64(11):1361-8. doi: 10.1099/jmm.0.000174. Epub 2015 Sep 23.

Pseudomonas aeruginosa flagellin as an adjuvant: superiority of a conjugated form of flagellin versus a mixture with a human immunodeficiency virus type 1 vaccine candidate in the induction of immune responses.

Author information

1
1​ Department of Biology, Faculty of Sciences, Qom branch of Islamic Azad University, Qom, Iran.
2
2​ Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
3
3​ Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
4
4​ Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
5
5​ Department of Medical Virology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
6
6​ Molecular Pathology Laboratory, Cancer Molecular Pathology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
7
7​ Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran.
8
5​ Department of Medical Virology, Razi Vaccine and Serum Research Institute, Karaj, Iran 8​ Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
9
9​ Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.

Abstract

In the present study, the adjuvant activity of flagellin was compared, in the conjugated and mixed forms, against a peptide vaccine from human immunodeficiency virus type 1 (HIV-1) p24-Nef vaccine candidate. Mice were immunized with the HIV-1 p24-Nef peptide with flagellin in both conjugated and mixed forms. Lymphocyte proliferation, CTL activity, and IL-4 and IFN-γ cytokines were evaluated by bromodeoxyuridine, carboxyfluorescein succinimidyl ester and ELISA methods, respectively. At the same time, the frequency of IFN-γ-producing T-lymphocytes, as well as total and isotype-specific antibodies, were assessed by ELISPOT and indirect ELISA, respectively. Our experimental results showed that the immunized mice with the HIV-1 p24-Nef conjugated or mixed forms of flagellin led to increases in the proliferative responses and Th1 cytokine pattern. The conjugated form of vaccine led to increased CTL activity and a Th1 cytokine pattern of immune responses, as well as an IgM isotype of humoral responses in comparison with the mixed form. The flagellin-conjugated vaccine seems to be more potent in increasing vaccine immunogenicity.

PMID:
26404477
DOI:
10.1099/jmm.0.000174
[Indexed for MEDLINE]

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