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Nature. 2015 Sep 24;525(7570):469-78. doi: 10.1038/nature15515.

Hallmarks of pluripotency.

Author information

1
Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Children's Hospital Boston, and Dana-Farber Cancer Institute; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
2
Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA.
3
Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA.
4
Department of Biomedical Informatics, Harvard Medical School, Boston, Massachusetts 02115, USA.
5
School of Mathematical Sciences and Center for Statistical Science, Peking University, Beijing 100871, China.
6
Stem Cell Unit, Department of Genetics, Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
7
College of Life Sciences and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
8
Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Boston, Massachusetts 02114, USA.
9
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
10
Medical Research Council Clinical Sciences Centre, Imperial College London, London W12 0NN, United Kingdom.
11
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California 94305, USA.
12
South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
13
The Hospital for Sick Children Research Institute, Toronto, Ontario ON M5G 0A4, Canada.

Abstract

Stem cells self-renew and generate specialized progeny through differentiation, but vary in the range of cells and tissues they generate, a property called developmental potency. Pluripotent stem cells produce all cells of an organism, while multipotent or unipotent stem cells regenerate only specific lineages or tissues. Defining stem-cell potency relies upon functional assays and diagnostic transcriptional, epigenetic and metabolic states. Here we describe functional and molecular hallmarks of pluripotent stem cells, propose a checklist for their evaluation, and illustrate how forensic genomics can validate their provenance.

PMID:
26399828
DOI:
10.1038/nature15515
[Indexed for MEDLINE]

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