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J Cell Biochem. 2016 Apr;117(4):978-87. doi: 10.1002/jcb.25383. Epub 2016 Feb 5.

Anti-Inflammatory Effect of Ascochlorin in LPS-Stimulated RAW 264.7 Macrophage Cells Is Accompanied With the Down-Regulation of iNOS, COX-2 and Proinflammatory Cytokines Through NF-κB, ERK1/2, and p38 Signaling Pathway.

Author information

1
Molecular and Cellular Glycobiology Laboratory, Department of Biological Science, SungKyunKwan University, Suwon City, Kyunggi-Do, Republic of Korea.
2
Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan City, Gyeongsangnam-Do, Republic of Korea.
3
Research Institute of Biomedical Engineering, Department of Medicine, School of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
4
Faculty of Pharmacy, Yeungnam University, Kyungsan, Republic of Korea.
5
Faculty of Medicinal Biotechnology, Dong-A University, Busan, Republic of Korea.
6
Bio-Medical Research Institute, Kyungpook National University Hospital, Daegu, Republic of Korea.
7
Magae Bioscience Institute, 49-4 Fujimidai, Tsukuba, 300-1263, Japan.

Abstract

A natural compound C23 H32 O4 Cl, ascochlorin (ASC) isolated from an incomplete fungus, Ascochyta viciae has been known to have several biological activities as an antibiotic, antifungal, anti-cancer, anti-hypolipidemic, and anti-hypertension agent. In this study, anti-inflammatory activity has been investigated in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells, since ASC has not been observed on the inflammatory events. The present study has clearly shown that ASC (1-50 μM) significantly suppressed the production of nitric oxide (NO) and prostaglandin E2 (PGE2 ) and decreased the gene expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Moreover, ASC inhibited the mRNA expression and the protein secretion of interleukin (IL)-1β and IL-6 but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 macrophage cells. In addition, ASC suppressed nuclear translocation and DNA binding affinity of nuclear factor-κB (NF-κB). Furthermore, ASC down-regulated phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) and p-p38. These results demonstrate that ASC exhibits anti-inflammatory effects in RAW 264.7 macrophage cells.

KEYWORDS:

ASCOCHLORIN; INFLAMMATION; LPS; RAW 264.7 MACROPHAGE CELLS

PMID:
26399466
DOI:
10.1002/jcb.25383
[Indexed for MEDLINE]

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