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Biosens Bioelectron. 2016 Mar 15;77:107-10. doi: 10.1016/j.bios.2015.09.031. Epub 2015 Sep 15.

A molecular beacon microarray based on a quantum dot label for detecting single nucleotide polymorphisms.

Author information

1
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
2
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: sunqj@seu.edu.cn.

Abstract

In this work, we report the application of streptavidin-coated quantum dot (strAV-QD) in molecular beacon (MB) microarray assays by using the strAV-QD to label the immobilized MB, avoiding target labeling and meanwhile obviating the use of amplification. The MBs are stem-loop structured oligodeoxynucleotides, modified with a thiol and a biotin at two terminals of the stem. With the strAV-QD labeling an "opened" MB rather than a "closed" MB via streptavidin-biotin reaction, a sensitive and specific detection of label-free target DNA sequence is demonstrated by the MB microarray, with a signal-to-background ratio of 8. The immobilized MBs can be perfectly regenerated, allowing the reuse of the microarray. The MB microarray also is able to detect single nucleotide polymorphisms, exhibiting genotype-dependent fluorescence signals. It is demonstrated that the MB microarray can perform as a 4-to-2 encoder, compressing the genotype information into two outputs.

KEYWORDS:

DNA microarray; Encoder; Molecular beacon; Quantum dots; Single nucleotide polymorphism

PMID:
26397421
DOI:
10.1016/j.bios.2015.09.031
[Indexed for MEDLINE]

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