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J Biomed Phys Eng. 2015 Sep 1;5(3):105-14. eCollection 2015 Sep.

Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells.

Author information

1
Radiobiology department, School of paramedical sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
2
Medical Physics Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran ; The Center for Research on Protection against Ionizing and Non-ionizing Radiation, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
3
Telecommunication Department, School of Engineering, Shiraz University, Shiraz, Iran.
4
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
5
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
6
The Center for Research on Protection against Ionizing and Non-ionizing Radiation, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

BACKGROUND:

Despite numerous studies over a decade, it still remains controversial about the biological effects of RF EMF emitted by mobile phone telephony.

OBJECTIVE:

Here we investigated the effect of 900 MHz GSM on the induction of oxidative stress and the level of intracellular reactive oxygen species (ROS) in human mononuclear cells, monocytes and lymphocytes as defence system cells.

METHOD:

6 ml Peripheral Blood samples were obtained from 13 healthy volunteers (21-30 year-old). Each sample was devided into 2 groups: one was exposed RF radiation emitted from a mobile phone simulator for 2 hour and the other used as control group which was not exposed to any fields. After that, mononuclear cells were isolated from peripheral blood by density gradient centrifugation in Ficoll-Paque. The intracellular ROS content in monocytes and lymphocytes was measured by the CM-H2DCFDA fluorescence probe using flowcytometry technique.

RESULTS:

Our results showed significant increase in  ROS production after exposure in population rich in monocytes. This effect was not significant in population rich in lymphocytes in comparison with non exposed cells.

CONCLUSION:

The results obtained in this study clearly showed the oxidative stress induction capability of RF electromagnetic field in the portion of PBMCs mostly in monocytes, like the case of exposure to micro organisms, although the advantages or disadvantages of this effect should be evaluated.

KEYWORDS:

Electromagnetic Radiation; Human Peripheral Blood Mononuclear Cells; Mobile phone; Radiofrequency (RF); Reactive Oxygen Species (ROS)

PMID:
26396966
PMCID:
PMC4576871

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