Large-scale live imaging of adult neural stem cells in their endogenous niche

Development. 2015 Oct 15;142(20):3592-600. doi: 10.1242/dev.123018. Epub 2015 Sep 22.

Abstract

Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.

Keywords: Live imaging; Multiphoton microscopy; Neural stem cell; Pallium; SHG/THG; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Brain / metabolism*
  • Cell Lineage
  • Cell Proliferation
  • Green Fluorescent Proteins / metabolism
  • Hydrogen-Ion Concentration
  • Image Processing, Computer-Assisted / methods
  • Luminescent Proteins / metabolism
  • Microscopy, Fluorescence
  • Neural Stem Cells / cytology*
  • Neuroimaging / methods*
  • Red Fluorescent Protein
  • Stem Cell Niche*
  • Telencephalon
  • Temperature
  • Transgenes
  • Zebrafish

Substances

  • Luminescent Proteins
  • Green Fluorescent Proteins