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Development. 2015 Oct 15;142(20):3592-600. doi: 10.1242/dev.123018. Epub 2015 Sep 22.

Large-scale live imaging of adult neural stem cells in their endogenous niche.

Author information

1
Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France.
2
Laboratory for Optics and Biosciences, École Polytechnique, Centre National de la Recherche Scientifique (UMR 7645) and Institut National de la Santé et de la Recherche Médicale (U1182), Palaiseau 91128, France.
3
Laboratory for Optics and Biosciences, École Polytechnique, Centre National de la Recherche Scientifique (UMR 7645) and Institut National de la Santé et de la Recherche Médicale (U1182), Palaiseau 91128, France emmanuel.beaurepaire@polytechnique.edu bally-cuif@inaf.cnrs-gif.fr.
4
Paris-Saclay Institute for Neuroscience, CNRS UMR9197 - Université Paris-Sud, Team Zebrafish Neurogenetics, Avenue de la Terrasse, Building 5, Gif-sur-Yvette F-91198, France emmanuel.beaurepaire@polytechnique.edu bally-cuif@inaf.cnrs-gif.fr.

Abstract

Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.

KEYWORDS:

Live imaging; Multiphoton microscopy; Neural stem cell; Pallium; SHG/THG; Zebrafish

PMID:
26395477
PMCID:
PMC4631764
DOI:
10.1242/dev.123018
[Indexed for MEDLINE]
Free PMC Article

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