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Mol Neurobiol. 2016 Oct;53(8):5103-17. doi: 10.1007/s12035-015-9433-6. Epub 2015 Sep 22.

HDAC3 Is Required for Posterior Lateral Line Development in Zebrafish.

He Y1, Wang Z2,3, Sun S4, Tang D1, Li W1, Chai R5, Li H6,7,8,9.

Author information

1
Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China.
2
Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China. fjswzm2015@126.com.
3
Key Laboratory of Hearing Medicine of National Health and Family Planning Commission, Shanghai, People's Republic of China. fjswzm2015@126.com.
4
Key Laboratory of Metabolism and Molecular Medicine, the Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai, People's Republic of China.
5
Co-innovation Center of Neuroregeneration, Key Laboratory for Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing, Jiangsu, 210096, People's Republic of China.
6
Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, People's Republic of China. hwli@shmu.edu.cn.
7
Key Laboratory of Hearing Medicine of National Health and Family Planning Commission, Shanghai, People's Republic of China. hwli@shmu.edu.cn.
8
State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, People's Republic of China. hwli@shmu.edu.cn.
9
Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, People's Republic of China. hwli@shmu.edu.cn.

Abstract

Histone deacetylases (HDACs) are involved in multiple developmental processes, but their functions in the development of mechanosensory organs are largely unknown. In the present study, we report the presence of HDAC3 in the zebrafish posterior lateral line primordium and newly deposited neuromasts. We used morpholinos to show that HDAC3 knockdown severely disrupts the development of the posterior lateral line and reduces the numbers of neuromasts and sensory hair cells within these organs. In HDAC3 morphants, we also observed decreased cell proliferation and increased apoptosis, which might lead to these defects. Finally, we show that HDAC3 deficiency results in attenuated Fgf signaling in the migrating primordium. In situ hybridizations indicate aberrant expression patterns of Notch signaling pathway genes in HDAC3 morphants. In addition, inhibition of HDAC3 function diminishes cxcr7b and alters cxcl12a expression in the migrating primordium. Our results indicate that HDAC3 plays a crucial role in regulating posterior lateral line (PLL) formation and provide evidence for epigenetic regulation in auditory organ development.

KEYWORDS:

Fgf signaling; HDAC3; Posterior lateral line; Zebrafish

PMID:
26395281
DOI:
10.1007/s12035-015-9433-6
[Indexed for MEDLINE]

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