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Development. 2015 Sep 15;142(18):3210-21. doi: 10.1242/dev.124289.

Hsa-miR-30d, secreted by the human endometrium, is taken up by the pre-implantation embryo and might modify its transcriptome.

Author information

1
Fundación Instituto Valenciano de Infertilidad (FIVI), Department of Obstetrics and Gynecology, Universitat de València, Instituto Universitario IVI/INCLIVA, 46980 Valencia, Spain felipe.vilella@ivi.es.
2
Fundación Instituto Valenciano de Infertilidad (FIVI), Department of Obstetrics and Gynecology, Universitat de València, Instituto Universitario IVI/INCLIVA, 46980 Valencia, Spain.
3
Departamento de Biología Celular y Parasitología, Facultad de Farmacia, Universitat de València, 46100 Burjassot (Valencia), Spain.
4
Fundación Instituto Valenciano de Infertilidad (FIVI), Department of Obstetrics and Gynecology, Universitat de València, Instituto Universitario IVI/INCLIVA, 46980 Valencia, Spain Department of Obstetrics and Gynecology, School of Medicine, Stanford University, Palo Alto, CA 94304, USA.

Abstract

During embryo implantation, the blastocyst interacts with and regulates the endometrium, and endometrial fluid secreted by the endometrial epithelium nurtures the embryo. Here, we propose that maternal microRNAs (miRNAs) might act as transcriptomic modifier of the pre-implantation embryo. Microarray profiling revealed that six of 27 specific, maternal miRNAs were differentially expressed in the human endometrial epithelium during the window of implantation--a brief phase of endometrial receptivity to the blastocyst--and were released into the endometrial fluid. Further investigation revealed that hsa-miR-30d, the expression levels of which were most significantly upregulated, was secreted as an exosome-associated molecule. Exosome-associated and free hsa-miR-30d was internalized by mouse embryos via the trophectoderm, resulting in an indirect overexpression of genes encoding for certain molecules involved in the murine embryonic adhesion phenomenon--Itgb3, Itga7 and Cdh5. Indeed, this finding was supported by evidence in vitro: treating murine embryos with miR-30d resulted in a notable increase in embryo adhesion. Our results suggest a model in which maternal endometrial miRNAs act as transcriptomic modifiers of the pre-implantation embryo.

KEYWORDS:

Embryo adhesion; Endometrial fluid; Has-miR-30d; Pre-implantation embryo

PMID:
26395145
DOI:
10.1242/dev.124289
[Indexed for MEDLINE]
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