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Biochim Biophys Acta. 2015 Dec;1854(12):1833-1841. doi: 10.1016/j.bbapap.2015.09.005. Epub 2015 Sep 25.

Selection of specific interactors from phage display library based on sea lamprey variable lymphocyte receptor sequences.

Author information

1
Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, 50-383 Wroclaw, Poland.
2
Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, 50-383 Wroclaw, Poland. Electronic address: otlewski@protein.pl.

Abstract

Variable lymphocyte receptors (VLRs) are non-immunoglobulin components of adaptive immunity in jawless vertebrates. These proteins composed of leucine-rich repeat modules offer some advantages over antibodies in target binding and therefore are attractive candidates for biotechnological applications. In this paper we report the design and characterization of a phage display library based on a previously proposed dVLR scaffold containing six LRR modules [Wezner-Ptasinska et al., 2011]. Our library was designed based on a consensus approach in which the randomization scheme reflects the frequencies of amino acids naturally occurring in respective positions responsible for antigen recognition. We demonstrate general applicability of the scaffold by selecting dVLRs specific for lysozyme and S100A7 protein with KD values in the micromolar range. The dVLR library could be used as a convenient alternative to antibodies for effective isolation of high affinity binders.

KEYWORDS:

Consensus approach; Phage library; VLR; Variable lymphocyte receptor

PMID:
26391289
DOI:
10.1016/j.bbapap.2015.09.005
[Indexed for MEDLINE]

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