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Sci Rep. 2015 Sep 21;5:14107. doi: 10.1038/srep14107.

Gene expression profiling of pre-eclamptic placentae by RNA sequencing.

Author information

1
Medical and Clinical Genetics, University of Helsinki and Helsinki University Hospital, Helsinki, FI-00014, Finland.
2
Research Programs Unit, Genome-Scale Biology and Institute of Biomedicine, University of Helsinki, Helsinki, FI-00014, Finland.
3
Molecular Neurology Research Program, University of Helsinki, Helsinki, FI-00014, Finland.
4
Folkhälsan Institute of Genetics, Helsinki, FI-000290, Finland.
5
Department of Biosciences and Nutrition, Center for Innovative Medicine, and Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden.
6
Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, FI-00014, Finland.
7
Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, FI-00014, Finland.

Abstract

Pre-eclampsia is a common and complex pregnancy disorder that often involves impaired placental development. In order to identify altered gene expression in pre-eclamptic placenta, we sequenced placental transcriptomes of nine pre-eclamptic and nine healthy pregnant women in pools of three. The differential gene expression was tested both by including all the pools in the analysis and by excluding some of the pools based on phenotypic characteristics. From these analyses, we identified altogether 53 differently expressed genes, a subset of which was validated by qPCR in 20 cases and 19 controls. Furthermore, we conducted pathway and functional analyses which revealed disturbed vascular function and immunological balance in pre-eclamptic placenta. Some of the genes identified in our study have been reported by numerous microarray studies (BHLHE40, FSTL3, HK2, HTRA4, LEP, PVRL4, SASH1, SIGLEC6), but many have been implicated in only few studies or have not previously been linked to pre-eclampsia (ARMS2, BTNL9, CCSAP, DIO2, FER1L4, HPSE, LOC100129345, LYN, MYO7B, NCMAP, NDRG1, NRIP1, PLIN2, SBSPON, SERPINB9, SH3BP5, TET3, TPBG, ZNF175). Several of the molecules produced by these genes may have a role in the pathogenesis of pre-eclampsia, and some could qualify as biomarkers for prediction or detection of this pregnancy complication.

PMID:
26388242
PMCID:
PMC4585671
DOI:
10.1038/srep14107
[Indexed for MEDLINE]
Free PMC Article

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