Format

Send to

Choose Destination
Int Immunopharmacol. 2015 Dec;29(2):701-707. doi: 10.1016/j.intimp.2015.09.006. Epub 2015 Sep 18.

Antitumor and immunomodulatory activity of genkwanin on colorectal cancer in the APC(Min/+) mice.

Author information

1
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210000, China.
3
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Tang Center of Herbal Medicine Research, Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA. Electronic address: zhangchunfeng67@163.com.
4
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: lifeicpu@163.com.
5
Tang Center of Herbal Medicine Research, Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.

Abstract

Colorectal cancer is the third most common malignant tumor with high morbidity and mortality. To evaluate the antitumor effect of genkwanin on colorectal cancer enhanced by western high-fat diet, we investigated the activity of genkwanin on HT-29 and SW-480 human colorectal cancer lines in vitro and on the APC(Min/+) mice in vivo. In a cell culture system, six different inflammatory cytokines obviously stimulated two cancer cells growth in a concentration-dependent manner, while genkwanin significantly inhibited HT-29 and SW-480 human colorectal cancer cells proliferation and inflammatory cytokine IL-8 secretion. In the APC(Min/+) mice, the body weights, spleen and thymus indexes and immunity cytokine secretions were significantly improved after oral administration 12.5 and 25mg/kg/day of genkwanin. Besides, the tumor multiplicity changes and inflammatory cytokine levels were markedly reduced in two genkwanin-treated groups. The dysplastic adenomatous changes were also obviously ameliorated in gut histopathology. Taken together, our results indicated that genkwanin had a better antitumor activity partly via enhancing host immunity and decreasing the inflammatory cytokine levels. Genkwanin may be an effective chemotherapeutic agent for the treatment of colorectal cancer.

KEYWORDS:

APC(Min/+) mice; Colorectal cancer; Genkwanin; Immunity organ index; Inflammatory cytokines

PMID:
26388189
DOI:
10.1016/j.intimp.2015.09.006
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center