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Biochem Pharmacol. 2015 Dec 1;98(3):502-10. doi: 10.1016/j.bcp.2015.09.008. Epub 2015 Sep 18.

First report on the pharmacokinetics of tramadol and O-desmethyltramadol in exhaled breath compared to plasma and oral fluid after a single oral dose.

Author information

1
Karolinska Institutet, Department of Laboratory Medicine, Division of Clinical Pharmacology, Stockholm, Sweden. Electronic address: markusr.meyer@gmx.de.
2
Karolinska Institutet, Department of Laboratory Medicine, Division of Clinical Pharmacology, Stockholm, Sweden; Karolinska University Laboratory, Department of Clinical Pharmacology, Stockholm, Sweden.
3
Karolinska University Laboratory, Department of Clinical Pharmacology, Stockholm, Sweden.

Abstract

Exhaled breath (EB) is a promising matrix for bioanalysis of non-volatiles and has been routinely implemented for drugs of abuse analysis. Nothing is known regarding the pharmacokinetics of therapeutics and their metabolites in EB. Therefore, we used tramadol as a model drug. Twelve volunteers received a single oral dose of tramadol and repeated sampling of EB, plasma, and oral fluid (OF) was done for 48 h using a particle filter device for EB and the Quantisal-device for OF. Samples were analyzed with LC-MS/MS and the pharmacokinetic correlations between matrices were investigated. The initial tramadol half-life in EB was shorter than in plasma but it reappeared in EB after 8-24 h. The ratio of O-desmethyltramadol to tramadol was considerably lower in EB and OF compared to plasma. This pilot study compared for the first time the pharmacokinetics of a therapeutic drug and active metabolite in different biomatrices including EB and demonstrated its potential for bioanalysis.

KEYWORDS:

Blood plasma; Exhaled breath; LC–MS/MS; Oral fluid; Pharmacokinetics; Tramadol

PMID:
26388171
DOI:
10.1016/j.bcp.2015.09.008
[Indexed for MEDLINE]

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