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Cell Rep. 2015 Oct 6;13(1):23-30. doi: 10.1016/j.celrep.2015.08.061. Epub 2015 Sep 17.

ΔN-TRPV1: A Molecular Co-detector of Body Temperature and Osmotic Stress.

Author information

1
Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, Canada.
2
Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, Canada; Institut du Cerveau et de la Moelle Épinière, Hôpital Pitié Salpêtrière, 47 Boulevard de l'Hôpital, 75013 Paris, France.
3
Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, Canada; Neurocentre Magendie, INSERM U862, 146 Rue Léo Saignat, 33077 Bordeaux, France.
4
Department of Neurology and Center for Translational Neuroscience, 201G Bryan Research Building, Box 2900, Duke University Medical Center, Durham, NC 27710, USA.
5
Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, Canada. Electronic address: charles.bourque@mcgill.ca.

Abstract

Thirst and antidiuretic hormone secretion occur during hyperthermia or hypertonicity to preserve body hydration. These vital responses are triggered when hypothalamic osmoregulatory neurons become depolarized by ion channels encoded by an unknown product of the transient receptor potential vanilloid-1 gene (Trpv1). Here, we show that rodent osmoregulatory neurons express a transcript of Trpv1 that mediates the selective translation of a TRPV1 variant that lacks a significant portion of the channel's amino terminus (ΔN-TRPV1). The mRNA transcript encoding this variant (Trpv1dn) is widely expressed in the brains of osmoregulating vertebrates, including the human hypothalamus. Transfection of Trpv1dn into heterologous cells induced the expression of ion channels that could be activated by either hypertonicity or by heating in the physiological range. Moreover, expression of Trpv1dn rescued the osmosensory and thermosensory responses of single hypothalamic neurons obtained from Trpv1 knockout mice. ΔN-TRPV1 is therefore a co-detector of core body temperature and fluid tonicity.

PMID:
26387947
DOI:
10.1016/j.celrep.2015.08.061
[Indexed for MEDLINE]
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