Format

Send to

Choose Destination
J Nutr Biochem. 2016 Jan;27:53-60. doi: 10.1016/j.jnutbio.2015.08.017. Epub 2015 Aug 24.

Chlorogenic acid improves ex vivo vessel function and protects endothelial cells against HOCl-induced oxidative damage, via increased production of nitric oxide and induction of Hmox-1.

Author information

1
School of Medicine and Pharmacology, University of Western Australia, Level 4, Medical Research Foundation Building, Rear 50 Murray St., Perth, WA, 6000, Australia.
2
School of Medicine and Pharmacology, University of Western Australia, Level 4, Medical Research Foundation Building, Rear 50 Murray St., Perth, WA, 6000, Australia. Electronic address: kevin.croft@uwa.edu.au.
3
School of Medicine and Pharmacology, University of Western Australia, Level 4, Medical Research Foundation Building, Rear 50 Murray St., Perth, WA, 6000, Australia; School of Biomedical Science & Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia.

Abstract

Dietary polyphenols are potential contributors toward improved cardiovascular health. Coffee is one of the richest sources of dietary polyphenols in a coffee-drinking population, the most abundant form being chlorogenic acid (CGA). Endothelial dysfunction is an early and major risk factor for cardiovascular disease. Nitric oxide (NO) is a key factor in regulation of endothelial function. Heme oxygenase-1 (Hmox-1), an inducible isoform of heme oxygenase that is produced in response to stressors such as oxidative stress, may also play a role in vascular protection. The aim of this study was to investigate the effect of CGA on endothelial function with oxidant-induced damage in isolated aortic rings from C57BL mice. We further examine the mechanism by investigating cell viability, activation of eNOS and induction of Hmox-1 in human aortic endothelial cells (HAECs). We found that pretreatment of isolated aortic rings with 10-μM CGA-protected vessels against HOCl-induced endothelial dysfunction (P<0.05). Pretreatment of cultured HAECs with 10-μM CGA increased endothelial cell viability following exposure to HOCl (P<0.05). Moreover, CGA increased NO production in HAECs in a dose-dependent manner, peaking at 6 h (P<0.05). CGA at 5 μM and 10 μM increased eNOS dimerization at 6 h and induced Hmox-1 protein expression at 6 h and 24 h in HAECs. These results are consistent with the cardiovascular protective effects of coffee polyphenols and demonstrate that CGA can protect vessels and cultured endothelial cells against oxidant-induced damage. The mechanism behind the beneficial effect of CGA appears to be in part via increased production of NO and induction of Hmox-1.

KEYWORDS:

Chlorogenic acid; Endothelial function; Heme oxygenase-1; Nitric oxide; Oxidative stress; Polyphenols

PMID:
26386740
DOI:
10.1016/j.jnutbio.2015.08.017
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center