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J Mol Neurosci. 2016 Jan;58(1):39-45. doi: 10.1007/s12031-015-0648-9. Epub 2015 Sep 18.

Ectopic Muscle Expression of Neurotrophic Factors Improves Recovery After Nerve Injury.

Author information

1
Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv, Israel.
2
Felsenstein Medical Research Center, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
3
Department of Neurology, Rabin Medical Center, Petach Tikva, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
4
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
5
Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv, Israel. danioffen@gmail.com.
6
Felsenstein Medical Research Center, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. danioffen@gmail.com.
7
The Neuroscience Laboratory, Felsenstein Medical Research Center, Rabin Medical Center, 49100, Petah Tikva, Israel. danioffen@gmail.com.

Abstract

Sciatic nerve damage is a common medical problem. The main causes include direct trauma, prolonged external nerve compression, and pressure from disk herniation. Possible complications include leg numbness and the loss of motor control. In mild cases, conservative treatment is feasible. However, following severe injury, recovery may not be possible. Neuronal regeneration, survival, and maintenance can be achieved by neurotrophic factors (NTFs). In this study, we examined the potency of combining brain-derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) on the recovery of motor neuron function after crush injury of the sciatic nerve. We show that combined NTF application increases the survival of motor neurons exposed to a hypoxic environment. The ectopic expression of NTFs in the injured muscle improves the recovery of the sciatic nerve after crush injury. A significantly faster recovery of compound muscle action potential (CMAP) amplitude and conduction velocity is observed after muscle injections of viral vectors expressing a mixture of the four NTF genes. Our findings suggest a rationale for using genetic treatment with a combination of NTF-expressing vectors, as a potential therapeutic approach for severe peripheral nerve injury.

KEYWORDS:

Compound muscle action potential (CMAP); Conduction velocity; Nerve injury; Neurotrophic factors

PMID:
26385386
DOI:
10.1007/s12031-015-0648-9
[Indexed for MEDLINE]

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