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Biochim Biophys Acta. 2016 May;1863(5):1038-48. doi: 10.1016/j.bbamcr.2015.09.015. Epub 2015 Sep 16.

Biogenesis, maintenance and dynamics of glycosomes in trypanosomatid parasites.

Author information

1
Systems Bioinformatics, Vrije Universiteit Amsterdam, The Netherlands.
2
Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, Venezuela.
3
Federal University of Minas Gerais, Laboratory of Immunoregulation of Infectious Diseases, Department of Biochemistry and Immunology, Institute for Biological Sciences, Belo Horizonte, Brazil.
4
Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida, Venezuela; Centre for Translational and Chemical Biology, Institute of Structural and Molecular Biology, School of Biological Sciences, University of Edinburgh, United Kingdom. Electronic address: paul.michels@ed.ac.uk.

Abstract

Peroxisomes of organisms belonging to the protist group Kinetoplastea, which include trypanosomatid parasites of the genera Trypanosoma and Leishmania, are unique in playing a crucial role in glycolysis and other parts of intermediary metabolism. They sequester the majority of the glycolytic enzymes and hence are called glycosomes. Their glycosomal enzyme content can vary strongly, particularly quantitatively, between different trypanosomatid species, and within each species during its life cycle. Turnover of glycosomes by autophagy of redundant ones and biogenesis of a new population of organelles play a pivotal role in the efficient adaptation of the glycosomal metabolic repertoire to the sudden, major nutritional changes encountered during the transitions in their life cycle. The overall mechanism of glycosome biogenesis is similar to that of peroxisomes in other organisms, but the homologous peroxins involved display low sequence conservation as well as variations in motifs mediating crucial protein-protein interactions in the process. The correct compartmentalisation of enzymes is essential for the regulation of the trypanosomatids' metabolism and consequently for their viability. For Trypanosoma brucei it was shown that glycosomes also play a crucial role in its life-cycle regulation: a crucial developmental control switch involves the translocation of a protein phosphatase from the cytosol into the organelles. Many glycosomal proteins are differentially phosphorylated in different life-cycle stages, possibly indicative of regulation of enzyme activities as an additional means to adapt the metabolic network to the different environmental conditions encountered.

KEYWORDS:

Biogenesis; Glycolysis; Glycosomes; Life-cycle differentiation; Trypanosomatid parasites; Turnover

PMID:
26384872
DOI:
10.1016/j.bbamcr.2015.09.015
[Indexed for MEDLINE]
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