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J Mech Behav Biomed Mater. 2016 Jan;53:312-319. doi: 10.1016/j.jmbbm.2015.08.038. Epub 2015 Sep 8.

Improved osseointegration of long-term stored SLA implant by hydrothermal sterilization.

Author information

1
School of Material Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, China.
2
School of Material Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, China. Electronic address: linly311@163.com.
3
Energy and Nuclear Research Institute, Material Science and Technology Center, Av. Prof. Lineu Prestes, 2242-Cidade Universitária, São Paulo 05508-000, Brazil.

Abstract

The sandblasted, large-grit and acid-etched (SLA) surface is easy to be contaminated during storage and its surface chemical state is usually changed by different sterilization methods. This causes an undesirable increase in surface hydrophobicity and results in osseointegration degradation. To overcome this problem, a low temperature hydrothermal (HT) sterilization method was proposed in this study. Briefly, 4 weeks-stored pure titanium SLA specimens were sterilized using a sealed glass bottle with pure water in an autoclave set at 121 °C for 20 min. Results showed that, stored SLA specimens were superhydrophobic before and after conventional autoclaving, whereas, HT sterilization decontaminated and endowed stored SLA surface with superhydrophilicity. Osteoblast spreading was greatly enhanced, ALP expression was upgraded and bone nodule formation was obviously promoted on HT sterilized specimens compared with autoclaved ones. More bone formation around HT sterilized specimens was observed and HT sterilization increased bonding strength of implant to bone by 95% and 127% after 2 and 4 weeks of healing, respectively. The simple, feasible HT sterilization restored osseointegration of SLA implant while diminishing recontamination as much as possible. Therefore, it is proposed as a standard sterilization method for implant practitioners and researches.

KEYWORDS:

Autoclaving; Hydrothermal sterilization; Osseointegration; SLA implant; Superhydrophilicity

PMID:
26382972
DOI:
10.1016/j.jmbbm.2015.08.038
[Indexed for MEDLINE]

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