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Nat Commun. 2015 Sep 18;6:8189. doi: 10.1038/ncomms9189.

Telomere maintenance through recruitment of internal genomic regions.

Author information

1
Department of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Korea.
2
Terry Fox Laboratory, BC Cancer Agency, Vancouver, Canada V5Z 1L3.
3
Department of Biological Sciences, Bioinformatics Institute, BIO-MAX, Seoul National University, Seoul 08826, Korea.
4
Research Institute, National Cancer Center, Goyang, Gyeonggi 10408, Korea.
5
Department of Biophysics and Chemical Biology, Seoul National University, Seoul 08826, Korea.

Abstract

Cells surviving crisis are often tumorigenic and their telomeres are commonly maintained through the reactivation of telomerase. However, surviving cells occasionally activate a recombination-based mechanism called alternative lengthening of telomeres (ALT). Here we establish stably maintained survivors in telomerase-deleted Caenorhabditis elegans that escape from sterility by activating ALT. ALT survivors trans-duplicate an internal genomic region, which is already cis-duplicated to chromosome ends, across the telomeres of all chromosomes. These 'Template for ALT' (TALT) regions consist of a block of genomic DNA flanked by telomere-like sequences, and are different between two genetic background. We establish a model that an ancestral duplication of a donor TALT region to a proximal telomere region forms a genomic reservoir ready to be incorporated into telomeres on ALT activation.

PMID:
26382656
PMCID:
PMC4595603
DOI:
10.1038/ncomms9189
[Indexed for MEDLINE]
Free PMC Article

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