Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170

Carbohydr Res. 2015 Nov 19:417:11-4. doi: 10.1016/j.carres.2015.08.013. Epub 2015 Aug 28.

Abstract

Enterotoxigenic Escherichia coli are causative agents of diarrhea in humans as well as animals, and E.coli O170 belongs to this virotype. Upon mild acid degradation of the lipopolysaccharide of E.coli O170, the branched O-polysaccharide chain was partially cleaved at β-d-glactofuranosidic linkages to give multiple products, including a linear tetrasaccharide and oligomers thereof. Studies of the acid degradation products and O-deacylated lipopolysaccharide by 1D and 2D (1)H and (13)C NMR spectroscopy enabled elucidation of the following O-polysaccharide structure: Functions of genes in the O-antigen biosynthesis gene cluster were tentatively assigned and found to be in agreement with the O-polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen gene cluster; O-polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Enterotoxigenic Escherichia coli / genetics*
  • Enterotoxigenic Escherichia coli / metabolism
  • Gene Expression Regulation, Bacterial*
  • Genes, Bacterial*
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Multigene Family
  • O Antigens / chemistry
  • O Antigens / genetics*
  • O Antigens / metabolism
  • Oligosaccharides / chemistry*

Substances

  • O Antigens
  • Oligosaccharides