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Biotechnol Biofuels. 2015 Sep 15;8:143. doi: 10.1186/s13068-015-0324-x. eCollection 2015.

Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.

Author information

1
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
2
Rizhao Entry-Exit Inspection and Quarantine Bureau, Rizhao, 276800 People's Republic of China.
3
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 People's Republic of China.

Abstract

BACKGROUND:

Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.

RESULTS:

The production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.

CONCLUSIONS:

The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.

KEYWORDS:

(2S,3S)-Butane-2,3-diol; Metabolic engineering; meso-Butane-2,3-diol dehydrogenase; α-Acetolactate synthase

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