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Biotechnol Biofuels. 2015 Sep 15;8:143. doi: 10.1186/s13068-015-0324-x. eCollection 2015.

Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.

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State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 People's Republic of China.
Rizhao Entry-Exit Inspection and Quarantine Bureau, Rizhao, 276800 People's Republic of China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 People's Republic of China.



Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.


The production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.


The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.


(2S,3S)-Butane-2,3-diol; Metabolic engineering; meso-Butane-2,3-diol dehydrogenase; α-Acetolactate synthase

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