Format

Send to

Choose Destination
FEMS Microbiol Lett. 2015 Nov;362(21). pii: fnv167. doi: 10.1093/femsle/fnv167. Epub 2015 Sep 15.

Characterizing and optimizing magnetosome production of Magnetospirillum sp. XM-1 isolated from Xi'an City Moat, China.

Author information

1
Paleomagnetism and Geochronology Laboratory, Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China France-China Bio-Mineralization and Nano-Structures Laboratory, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China.
2
State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100094, China.
3
Electron Microscope Laboratory, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China.
4
Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel-VUB, Brussels 1050, Belgium.
5
Paleomagnetism and Geochronology Laboratory, Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China France-China Bio-Mineralization and Nano-Structures Laboratory, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China Electron Microscope Laboratory, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China yxpan@mail.iggcas.ac.cn.

Abstract

Pure culture of magnetotactic bacteria is desirable to understand their physiology, evolution and biomineralization. Here, we report a new strain Magnetospirillum sp. XM-1 that was recently isolated and cultivated from the eutrophic city moat of Xi'an, China. Magnetosome biomineralization, crystallographic and magnetic properties of XM-1 were characterized by using a combination of transmission electron microscopy and rock magnetic methods. Cell growth and magnetite production was optimized by response surface methodology. We found that the Magnetospirillum strain XM-1 is different from the model strain Magnetospirillum magneticum AMB-1 in terms of magnetite magnetosomes, optimal growth temperature and nutrient requirements. Sodium succinate, sodium nitrate and ferric citrate are the three most significant factors associated with the optimization of cell growth and magnetosome production for XM-1.

KEYWORDS:

Magnetospirillum sp. XM-1; magnetic properties; magnetite formation; magnetosome production; magnetotactic bacteria; response surface methodology optimization

PMID:
26376913
DOI:
10.1093/femsle/fnv167
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center