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J Forensic Sci. 2016 Mar;61(2):430-438. doi: 10.1111/1556-4029.12950. Epub 2015 Sep 16.

Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y-chromosomal Short Tandem Repeat Analysis for Archeological Human Bones.

Oh CS1,2, Lee SD1,3, Shin KJ4, Shin DH1,2.

Author information

1
Institute of Forensic Science, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.
2
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.
3
Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-Gu, Seoul, 110-799, South Korea.
4
Department of Forensic Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-Gu, Seoul, 120-752, South Korea.

Abstract

The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom-designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y-STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom-designed primers). In this study, the authors established that the custom-designed primers offer a markedly improved success rate for obtainment of Y-STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay.

KEYWORDS:

AmpFℓSTR Yfiler PCR amplification kit; Joseon; Korea; Y-chromosomal short tandem repeat; Y-miniplex primer; ancient DNA; forensic science

PMID:
26375610
DOI:
10.1111/1556-4029.12950
[Indexed for MEDLINE]

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