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In Vitro Cell Dev Biol Anim. 2016 Jan;52(1):68-76. doi: 10.1007/s11626-015-9948-1. Epub 2015 Sep 15.

Umbilical cord mesenchymal stem cell-conditioned media prevent muscle atrophy by suppressing muscle atrophy-related proteins and ROS generation.

Author information

1
Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. chanmi319@gmail.com.
2
Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. treasure7744@naver.com.
3
Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. glena0204@naver.com.
4
Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. wlsgh3142@naver.com.
5
Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. kimzhun@gmail.com.
6
Department of Biotechnology, CHA University, 689 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, South Korea. yschoi@cha.ac.kr.

Abstract

The therapeutic potential of mesenchymal stem cell-conditioned medium (MSC-CM) has been reported with various types of disease models. Here, we examine the therapeutic effect of umbilical cord MSC-CM (UCMSC-CM) on muscle-related disease, using a dexamethasone (Dex)-induced muscle atrophy in vitro model. The expressions of muscle atrophy-related proteins (MuRF-1 and MAFbx) and muscle-specific proteins (desmin and myogenin) were evaluated by Western blot analysis. The level of production of reactive oxygen species (ROS) was determined using a 2',7'-dichlorofluorescein diacetate (DCFDA) dye assay. The expression of antioxidant enzymes (copper/zinc-superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPx-1), and catalase (CAT)) was verified by reverse transcription polymerase chain reaction (RT-PCR). When L6 cells were exposed to Dex, the expression of muscle atrophy-related proteins was increased by 50-70%, and the expression of muscle-specific proteins was in turn decreased by 23-40%. Conversely, when the L6 cells were co-treated with UCMSC-CM and Dex, the expression of muscle atrophy-related proteins was reduced in a UCMSC-CM dose-dependent manner and the expression of muscle-specific proteins was restored to near-normal levels. Moreover, ROS generation was effectively suppressed and the expression of antioxidant enzymes was recovered to a normal degree. These data imply that UCMSC-CM clearly has the potential to prevent muscle atrophy. Thus, our present study offers fundamental data on the potential treatment of muscle-related disease using UCMSC-CM.

KEYWORDS:

Dexamethasone; L6 skeletal muscle cell; Muscle atrophy; Umbilical cord mesenchymal stem cell (UCMSC); Umbilical cord mesenchymal stem cell-conditioned medium (UCMSC-CM)

PMID:
26373864
DOI:
10.1007/s11626-015-9948-1
[Indexed for MEDLINE]

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